2016 / Mar / mRNAseq on non-model organisms¶
Part of this workshop was given on Mar 30th, 2016, by C. Titus Brown. See the workshop organization page for more information, or contact Titus directly.
We have an EtherPad for sharing text and asking questions
Tutorials:
- Welcome!
- Non-model organisms and RNAseq
- Using Amazon Web Services
- Short read quality and trimming
- Running digital normalization
- Running the actual assembly
- Assembly statistics and evaluation
- BLASTing your assembled data
- Annotation of denovo transcriptome
- Quantification and Differential Expression
- Remapping your reads to your assembled transcriptome
- Miscellaneous advice
- More resources
- Miscellaneous questions
- Tips and Tricks for working with Remote Computers
Technical information¶
The github repository for this workshop is publicly available at https://github.com/ngs-docs/2016-mar-nonmodel.
LICENSE: This documentation and all textual/graphic site content is licensed under the Creative Commons - 0 License (CC0) -- fork @ github. Presentations (PPT/PDF) and PDFs are the property of their respective owners and are under the terms indicated within the presentation.